Thrombolytic Effects of Philippine Pit Viper (Trimeresurus flavomaculatus) Venom in Human Blood In Vitro and Ferric Chloride-Induced Cardiac Thrombosis on Swiss Webster Mice In Vivo

Introduction: Thrombosis is one of the leading causes mortality worldwide. Thrombolytic agents are used to reduce this burden. Studies pointed out that certain proteins in venom several snake species may have potential thrombolytic properties. Trimeresurus flavomaculatus, known as Philippine pit viper, found along damp localities Philippines. Venoms closely related been shown exhibit effects vitro and vivo. However, no extensive studies yet conducted about effect T. flavomaculatus venom. Thus, two-phased study aimed determine on human blood ferric chloride-induced cardiac thrombosis mice. Methodology: Phase 1 was done using clot lysis method measure activity vitro. Venom dilutions 3:4, 1:2, 2:3, 1:0, positive control (streptokinase), negative (normal saline solution) were inoculated different samples blood. 2 measured Ferric chloride-saturated filter paper applied over wall for induction thrombus formation. 1:64, 1:16, 1:4, 1:1, then injected through dorsal tail vein After hour, tissues excised histologic examination. Results: results showed had significant Dilutions 1:0 3:4 differences with streptokinase vivo at 1:64 dilutions. Conclusion: The indicated has a both